Sambrook Manual
General description The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. In this new edition, authors Joseph Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. Handsomely redesigned and presented in new bindings of proven durability, this three-volume work is essential for everyone using today’s biomolecular techniques.
Sambrook Manor Shropshire Uk
The opening chapters describe essential techniques, some well-established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small. These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing. The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein-protein interactions. The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information.
As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved. Table of Contents Chapter 1. Plasmids and Their Usefulness in Molecular Cloning Chapter 2. Bacteriophage lambda and Its Vectors Chapter 3. Working with Bacteriophage M13 Vectors Chapter 4. Working with High-capacity Vectors Chapter 5.
Gel Electrophoresis of DNA and Pulsed-field Agarose Gel Electrophoresis Chapter 6. Preparation and Analysis of Eukaryotic Genomic DNA Chapter 7. Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells Chapter 8. In vitro Amplification of DNA by the Polymerase Chain Reaction Chapter 9. Preparation of Radiolabeled DNA and RNA Probes Chapter 10. Working with Synthetic Oligonucleotide Probes Chapter 11. Preparation of cDNA Libraries and Gene Identification Chapter 12.
DNA Sequencing Chapter 13. Mutagenesis Chapter 14.
Sambrook Lab Manual
Screening Expression Libraries Chapter 15. Expression of Cloned Genes in Escherichia coli Chapter 16. Introducing Cloned Genes into Cultured Mammalian Cells Chapter 17. Analysis of Gene Expression in Cultured Mammalian Cells Chapter 18. Protein Interaction Technologies Appendices.
Sambrook Laboratory Manual
Molecular Cloning: A Laboratory Manual ( Fourth Edition) Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
Molecular Cloning, Fourth Edition, by the celebrated founding author Joe Sambrook and new co-author, the distinguished HHMI investigator Michael Green, preserves the highly praised detail and clarity of previous editions and includes specific chapters and protocols commissioned for the book from expert practitioners at Yale, U Mass, Rockefeller University, Texas Tech, Cold Spring Harbor Laboratory, Washington University, and other leading institutions. The theoretical and historical underpinnings of techniques are prominent features of the presentation throughout, information that does much to help trouble-shoot experimental problems.
Linhai 300 classic 4x4 service manual. • Αποστολή με Courier σε Ειδικές τιμές. • Μεταφόρτωση σε συνεργαζόμενο πρακτορείο (από Αθήνα). • Δωρεάν Μεταφορά μέχρι Αθήνα με μεταφορική εταιρία.
For the fourth edition of this classic work, the content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories. Core chapters from the third edition have been revised to feature current strategies and approaches to the preparation and cloning of nucleic acids, gene transfer, and expression analysis. They are augmented by 12 new chapters which show how DNA, RNA, and proteins should be prepared, evaluated, and manipulated, and how data generation and analysis can be handled. The new content includes methods for studying interactions between cellular components, such as microarrays, next-generation sequencing technologies, RNA interference, and epigenetic analysis using DNA methylation techniques and chromatin immunoprecipitation. To make sense of the wealth of data produced by these techniques, a bioinformatics chapter describes the use of analytical tools for comparing sequences of genes and proteins and identifying common expression patterns among sets of genes.
Building on thirty years of trust, reliability, and authority, the fourth edition of Molecular Cloning is the new gold standardthe one indispensable molecular biology laboratory manual and reference source.